Thus, Asp-161 in the flexible loop L1 of wild-type RecA determines the preference for single-stranded DNA-binding to the primary site and regulates the DNA-binding order in RecA-catalyzed recombinase reactions. We found that the D161A-replacement reduced this suppression, probably by allowing double-stranded DNA to bind preferentially and reversibly to the primary site. When RecA/Rad51-recombinases interact with double-stranded DNA before single-stranded DNA, homologous joint-formation is suppressed, likely by forming a dead-end product. This was confirmed by the effects of the Ala-replacement of Asp-161 (D161A), analyzed directly by gel-mobility shift assays and indirectly by DNA-dependent ATPase activity and SOS repressor cleavage. A comparison of the loop L1 structures in a DNA-free RecA crystal that we originally determined and in the reported DNA-bound active RecA crystals suggested that the aspartate at position 161 in loop L1 in DNA-free RecA prevented double-stranded, but not single-stranded, DNA-binding to the primary site. The underlying reason and the regulatory mechanism for this conserved binding order remain unknown. In homologous joint formation, ATP-bound RecA/Rad51-recombinases first bind single-stranded DNA at its primary site and then interact with double-stranded DNA at another site.
If you want something simple to help you manage all your podcasts subscriptions, organize your downloads so you can find the latest podcasts faster or simply want to find new information or perspective over a certain topic, then perhaps you can consider giving Recast a try.In all organisms, RecA-family recombinases catalyze homologous joint formation in homologous genetic recombination, which is essential for genome stability and diversification. In short, the program allows you to view and manage your subscriptions, as well as the Downloads effortlessly. A handy tool for managing all your podcastsĪ noteworthy feature of the application is that it can lend you a hand with organizing your podcasts more efficiently. You can use general terms to find more podcasts and perspectives on any given topic. In case you know exactly what you are looking for you can simply access the search tab and write the keywords or phrases directly. On a side note, in case the audio does not play at all, then the developer suggests that installing K-Lite Codec Pack Basic can solve the problem. Clicking on any podcasts enables you to set Alerts, Subscribe, Download, Play, add the playlist and grab the link.
While initially it displays a few suggestions, you can expand them via the More button located in the upper right area. The app does not come with a Home screen, but rather a Discover page where you can browse through all trending podcasts. The interface is user-friendly and designed as a dashboard with all the options displayed in the left panel.
DOWNLOAD RECASTO ARCHIVE
The program is lightweight and portable, so all you need to do to get started is decompress the archive in the desired location. Find anything fast via the Search function
Recast is a small tool designed to help you organize, find new podcasts as well as manage your subscriptions and downloads. Considering that they can cover just about every topic or genre, podcasts are considered a great source of entertainment and information at the same time.
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